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CHIP DELIVERY SYSTEM FOR
THERMO LTQ
Easy access to NanoSpray on your LTQ using the Agilent Brand proprietary Chip on your Thermo LTQ, Velos, Orbitrap or FT. Our inteface hardware and software system makes it possible.
Walk up and drop your chip in the slot. Click on "operate" and all the connections are made ! On-board enrichment column, analytical column, switching valve and ESI emitter tip. All in one micro-manufactured chip.
Agilent Chip for Proteomics: Rapid Protein ID, 43mm 300 A C18 chip w/40 nL traping column.
Click here for available chips from Agilent
Chip in spray mode, note the nano-spray bending slightly to the left .
Click Here for a quick Quotation
Click Here for a quick Quotation
The system configuration looks like this: There are two HPLC systems, which can be any brand but we think that Agilent 1100 and 1200 systems are awesome. There will be a Micro-capillary pumping system, that will load the On-Chip trapping column. After the trapping column is loaded, the On-Chip valve switches and a Nano pumping system takes over to elute the analyte off the trapping column and onto the On-Chip analytical column. Loading is done at around 4.0 ul/min, the analytical run done at 400 nl/min. Here's a list of recommended Agilent modules:
G2229A Nano Pump/Micro Degasser, G1382A Capillary Pump, and G1377A Micro Well-plate Sampler.
Of course a PC and Chemstation will be required to run the above modules. We have been very resourceful in setting up the PC system for joint LTQ, Chip Delivery System and HPLC control. Most PCs that come with the LTQ are basic, just enough to deal with the LTQ and the Data. Same situation for the Agilent HPLC Chemstation. So, we set up virtual connections (via ethernet) between the two PCs enabling you to run BOTH systems from one monitor and key pad. Of course if you purchase a new, powerful (quad processor) PC with lots of memory, we can install all the software and control on one PC.
The Mass Spec, HPLCs and the Chip Delivery System are all interconnected with remote start cables so full sequencing is straight forward, making automated runs easy.
Agilent Chips are available in many configurations. Custom chips are avaialble that can be made to your specification.
Click here to download a white paper with data included.
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Actual application of Chip Delivery System on LTQ FT with Agilent 1200 HPLC solvent system.
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Here is our interface connected to a Thermo LTQ Orbitrap Velos.
Screen Capture of control of both the Agilent 1200 HPLC system, the Chip Cube Box and the LTQ Run Control.
Click here for a white paper we have authored with assorted data.
Check out our Harvard Syringe pumps that we are using as the Nano "Analytical" pumping system. We have written a visual basic executable to program a true gradient and control the solvent delivery. We're still in the testing phase but intend to offer the OEM version of the Harvard pumps with our software by mid June 2010. Contact us by email for details, pricing (under $15k for the pair, including software) and delivery.
Here's a chromatograph of Digested IGG, eluted with a 10 minute gradient from 5% Acn to 70% Acn using the Harvard Syringe pumps and our gradient control program, on the Agilent Protein ID Chip.
Although we are not affiliated with Agilent Technologies, we are adapting their product to your LTQ, indluding the chips that they manufacture. Here are a few links to some application notes and other items of interest found on their web pages, regarding the Agilent Chip Cube and Agilent Chips:
Paper #1
Paper #2
Paper #3
Paper #4
Link to a really informative video of how the Chip Interface and Chip Works
Link to the Agilent Technologies "Chip Page"
"Agilent" is a trademark of Agilent Technologies
"Thermo", "LTQ" and "Velos" are trademarks of Thermo Fisher Scientific Inc.
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