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CHIP DELIVERY SYSTEM FOR
THERMO LTQ
Inject 10% of the amount of sample you currently are using and get results in 25% of the time using the Agilent Brand proprietary Chip with on-board enrichment column, analytical column, switching valve and ESI emitter tip. All in one micro-manufactured chip.
Agilent Chip for Proteomics
Rapid Protein ID, 43mm 300 A C18 chip w/40 nL traping column
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Chip in spray mode, note the nano-electrospray bending slightly to the left towards the LTQ mass spec entrance aperature.
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System Configuration looks like this: Two HPLC systems, can be any brand but we think that Agilent 1100 and 1200 systems are awesome. There will be a Micro-capillary pumping system, that will load the On-Chip trapping column. After the trapping column is loaded, the On-Chip valve switches and a Nano pumping system takes over to elute the analyte off the trapping column and onto the On-Chip analytical column. Loading is done at around 40 ul/min, the analytical run done at 50 nl/min to 200 nl/min. Here's a list of recommended Agilent modules:
G1379B Degasser (2), G2226A Nano Pump, G1371A Micro WPS (autosampler/injector), G1390B FC/ALS Therm (autosampler/sample cooler), G1376 Capillary Pump.
Of course a PC and Chemstation will be required to run the above modules. We have been very resourceful in setting up the PC system for joint LTQ, Chip Delivery System and HPLC control. Most PCs that come with the LTQ are basic, just enough to deal with the LTQ and the Data. Same situation for the Agilent HPLC Chemstation. So, we set up virtual connections (via ethernet) between the two PCs and are then able to run BOTH systems from one monitor and key pad.
The Mass Spec, HPLCs and the Chip Delivery System are all interconnected with remote start and stop cables so full sequencing is straight forward making automated runs easy.
Agilent Chips are available in many configurations. Custom chips are avaialble that can be made to your specifications.
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Actual application of Chip Delivery System on LTQ FT with Agilent 1200 HPLC solvent system.
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Screen Capture of control of both the Agilent 1200 HPLC system, the Chip Cube Box and the LTQ Run Control
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